Rat lipocortin I cDNA

Localization of lipocortin-1 in rat hypothalamus and pituitary gland.

Characterization of cDNA encoding human placental anticoagulant protein (PP4): homology with the lipocortin family.

A cDNA library prepared from human placenta was screened for sequences encoding the placental protein 4 (PP4). PP4 is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade. Partial amino acid sequence information from PP4-derived cyanogen bromide fragments was used to design three oligonucleotide pr...

Expression of human recombinant lipocortin I in a wheat-germ cell-free system and Xenopus oocytes. Lipocortin is not secreted.

Lipocortin I has been presumed to be synthesized and secreted in response to glucocorticoids yet the amino acid sequence of lipocortin I reveals no signal sequence typically necessary for proteins to enter the secretory pathway. The translocation of lipocortin I across membranes was analyzed in a cell-free system and in Xenopus oocytes. Based on the published sequence, the cDNA of human lipocor...

Lipocortin - 1 Is an Endogenous Inhibitor of Ischemic Damage in the Rat Brain By Jane

Lipocortin-1 (annexin-1) is an endogenous peptide with antiinflammatory properties . We have previously demonstrated lipocortin immunoreactivity in certain glial cells and neurons in the rat brain (Strijbos, P. J . L . M., F. J. H . Tilders, F. Carey, R . Forder, and N. J . Rothwell . 1990 . Brain Res. In press .), and have shown that an NH2-terminal fragment (1-188) of lipocortin-1 inhibits th...

Molecular cloning of cDNA for rat and human carbamyl phosphate synthetase I.

Recombinant plasmids with inserts complementary to the mRNA for carbamyl phosphate synthetase I were identified from a rat liver cDNA library by hybrid-selected mRNA translation. Four clones, the largest being 3100 base pairs, were identified for the rat liver enzyme. Using the rat liver cDNA as a probe, two homologous recombinant plasmids of approximately 1200 base pairs in length were isolate...